Journal:

Article Title: Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea

doi:

Figure Lengend Snippet: Titers of IgG2a antibodies reactive with CII in the arthritic joints and of non-GTP-fed and GTP-fed DBA/1 mice. Antibody titers were determined by an ELISA method using the mouse IgG isotyping kit (PharMingen) according to the manufacturer’s instructions. Joint extracts were prepared when clinical arthritis was evident, and the samples were run in triplicate for each analysis. Error bars indicate SEM.

Article Snippet: The wells were washed extensively, and then the alkaline phosphatase-conjugated goat anti-mouse IgG antibody (PharMingen) was applied, unbound antibody was washed, and the plates were developed by using the reagents of the mouse IgG isotype ELISA kit and the phosphatase substrate (Sigma).

Techniques: Enzyme-linked Immunosorbent Assay